A training course in “Gene Cloning and Recombinant Protein Expression” organized by the Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo was successfully held from 20th to 24th November 2023 at the IBMBB premises. The Director of the IBMBB, Prof Prasanna Galhena welcomed the participants on the first day of the training course. The training course was resourced by Prof Jagathpriya Weerasena, Professor in Molecular Biology, IBMBB, Dr Nilupa Gunaratna, Scientific Assistant, IBMBB, Ms Thulangi, IBMBB and Ms Sandali, IBMBB. The training course was conducted for young scientists, academics, undergraduate/postgraduate students and students awaiting higher studies in biological sciences. The training course provided an opportunity to perform the latest as well as commonly used methods in modern biotechnology such as PCR amplification and cloning, preparing recombinant constructs, transformation, protein expression and purification, protein crystallization, getting structural insights of proteins using bioinformatics/ computer graphics-based exercises etc. The training course was a great opportunity for the participants to broaden their knowledge in the field of Gene Cloning and Recombinant Protein Expression. The training course concluded with an open discussion, where the participants shared their views on the training course and both its contents and organization were greatly appreciated.