A training course in “Gene Cloning and Recombinant Protein Expression” organized by the Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo was successfully held from 25th to 29th November 2022 at the IBMBB premises. The training course was resourced by Prof Jagathpriya Weerasena, Professor in Molecular Biology, IBMBB, University of Colombo, Dr Sanjeewani Sooriyarachchi, Research Scientist, Institute of Cell and Molecular Biology, University of Uppsala, Uppsala, Sweden and Dr Nilupa Gunaratna, Scientific Assistant, IBMBB, University of Colombo, Ms. Thulangi Siriwardana and Ms. Sandali Geethma. The training course was conducted for the young scientists, academics, undergraduate/postgraduate students and students awaiting higher studies in biological sciences. The training course provided an opportunity to perform the latest as well as commonly used methods in modern biotechnology such as PCR amplification and cloning, preparing recombinant constructs, transformation, protein expression and purification, protein crystallization, getting structural insights of proteins using bioinformatics/ computer graphic-based exercises etc. The training course was a great opportunity for the participants to broaden their knowledge in the field of Gene Cloning and Recombinant Protein Expression. The training course concluded with an open discussion, where the participants shared their views on the training course and both its contents and organization were greatly appreciated.